Tissue-resident memory T cell reactivation by diverse antigen-presenting cells imparts distinct functional responses [II]

Temporal transcriptome profiling of TCR-reactivated (P14) and bystander-reactivated (OT-I) memory T cells in the lung and mediastinal lymph node based on Nur77-GFP reporter expression and CD98 expression Overall design: TCR-transgenic P14 and OT-I T cells were adoptively transferred to generate immune chimeras. At various timepoints post secondary influenza infection, P14 and OT-I T cells were sorted from the lung and lymph node based on their Nur77-GFP reporter or CD98 expression, in duplicate, and RNA-seq was performed.