Paired-end sequencing of the genome of Escherichia coli K-12 strain MG1655 using the Illumina Genome Analyzer IIx. E. coli paired-end sequencing project (2x100 bases)

E. coli K-12 holds a key position as a model organism in studies of molecular biology, biochemistry, genetics and biotechnology. A high-quality reference sequence of the genome of E. coli K-12 strain MG1655 has previously been generated using the Sanger dideoxy sequencing method (Reference sequence accession: NC_000913). We have re-sequenced the genome of the MG1655 strain using the Illumina Genome Analyzer IIx to provide a paired-end, 100 base sequence dataset for development of de novo sequence assembly algorithms or other downstream analysis tools. We generated a sequencing library with a median insert size of 500 bp following random fragmentation and gel fractionation of genomic DNA. Sequence reads were aligned to the reference genome using ELANDv2e. This study supersedes experiment ERX002508 and run ERR008613 under study ERP000092.