Entamoeba histolytica -bacterium interactions and the role of this interaction in intestinal pathogenesis

Entamoeba histolytica is a protist parasite causing amoebiasis. Trophozoites of E.histolytica multiplies in the large intestine occasionally causing disease. The large intestine in humans is populated by a number of different bacterial community and amoebic cells grow in their midst using some as food material. Several studies have shown relationship between bacteria and E. histolytica growth and virulence. We have investigated the possibility that there may be specific interaction of amoeba with different bacteria present in the gut environment by analysis of these bacteria with amoeba using 16SrRNA metagenomics. This was done by incubating bacteria isolated from human faecal material with E. histolytica and then identification of bacterial population isolated from amoebic cells (PP3715-1) using a 16 SrRNA based metagenomic approach and comparing this with the original stool metagenome (WS-1)