PERIOD and CRY2 in diurnal transcriptome regulation in the diamondback moth

To determine the rhythmic expression of genes potentially involved in regulating physiological and circadian rhythms in P. xylostella, we performed RNA-seq analysis on the head samples of P. xylostella collected every 4 hours. The G88(WT), Pxper-KO(M10), Pxcry2-KO(1.1) and PxperPxcry2-dKO(3.1) were subjected to a 4-day light-dark (14hL:10hD) cycle of 14L:10D. Male adult heads were collected at LD4 time points for RNA extraction, with two independent replicates per time point. Paired-end RNA-seq (2 x150 bp) sequencing strategy was performed using the Illumina NovaSeq 6 000 sequencer.