Single-cell Transcriptomes of RPE-specific AAV-GFP/AAV-PDGF-D mouse RPE-choroid complex. Single-cell Transcriptomes of RPE-specific AAV-GFP/AAV-PDGF-D mouse RPE-choroid complex

Purpose: PDGF-D is a potent angiogenic and immune-related factor. It has been demonstrated that PDGF-D play roles in the pathological process of ocular angiogenesis and degenerative diseases. In our previous study, we established a retinal pigment epithelial (RPE) cell-specifc AAV-PDGF-D/GFP mouse model to study PDGF-D's role in age-related macular degeneration (AMD). In this study, we utilized the single-cell transcriptiome seqeuncing technique to explore the cell types of immune cells attracted by PDGF-D overepxression using the same animal models, with emphases on the impact of PDGF-D overexpression on individual cell's behaviour. Overall design: scRNA-seq of mouse RPE-choroid complex received subretina injection of RPE-specific AAV-GFP and AAV-PDGF-D , which were collected after 4-weeks injection of AAVs.