RNAseq dataset.xlsx
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https://figshare.com/articles/dataset/RNAseq_dataset_xlsx/23634687
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RNA was extracted from the rhizosphere samples using a PowerSoil® RNA extraction kit (Qiagen Iberia S.L., Madrid, Spain) following the manufacturer's instructions and its amount was quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
For the RNAseq experiment, the quantity and quality of RNA were verified by the Genomics and Ultrasequencing Service Unit (University of Malaga) and subsequently sequenced using NextSeq550 equipment (Illumina). The raw reads and their subsequent processing were carried out by the Centre for Supercomputing and Bioinnovation (University of Malaga). Once the expression profile was calculated, the differentially expressed genes (P values < 0.05 and Log2 Fold Change > 1 or < −1) between the wild-type strain PcPCL1606 and ΔdarB in interaction with the roots of avocado plants were obtained in order to identify possible genes regulated by HPR.
创建时间:
2023-07-06



