soil metagenome under melting snowpack, bacterial and fungal amplicon sequencing reads

We sampled soils along a 200-m upland hillslope-to-riparian floodplain transect in a mountainous catchment at the East River Watershed, Colorado, USA (Hubbard et al. 2018). Soil samples were collected over a time-course that began after plant senescence in autumn, through snow accumulation during winter, during snowmelt, and through plant green-up in spring and early summer. We monitored soil temperature and moisture and quantified the dynamics of the soil microbial biomass pool as well as extractable N in soil that was measured as extractable soil nitrate (NO3-). Bacterial, archaeal, and fungal communities were identified over this seasonal time-course with amplicon sequencing (iTag) of marker genes (16S for Bacteria and Archaea, ITS for Fungi).