RNA-sequence facilitates analysis of mesenchymal stem cells treating with concanavalin A mice

Purpose: The goals of this study are to find the mechanisms of mesenchymal stem cells (MSC) ameliorating concanavalin A (Con A) induced inflammatory mice Methods: Liver tissues were harvested at 6h, 12h after Con A injected with/without MSC (n = 3 per group) for transcriptome profiling. Results: Filter the raw data obtained fromDNBSEQ-G400, and compare the filtered clean reads to the reference sequence. Quantitative analysis of known genes and new genes, differential expression analysis based on gene expression in different sample groups, GO function analysis, Pathway function analysis, cluster analysis, and protein interaction for the selected differentially expressed genes Network, transcription factor coding ability prediction and other in-depth mining analysis. Conclusions: Our study discoveres following processes were significantly affected: apoptosis, MAPK signaling pathway and cytokine-cytokine receptor interaction. Overall design: Total hepatocyte RNA was extracted for RNA-seq of 6h, 12h after Con A injected with/without MSC (n = 3 per group)