ChIP-seq analysis of normal and HBx transgenic mouse liver

A genome-wide integrated ChIP-seq analysis of the early stage of hepatocellular carcinoma development that induced by HBx. The HBV x (HBx) protein, which plays a critical role in the development of HCC, was shown to interact with several epigenetic factors, such as DNMT3A and HDAC1. Most HBx transgenic (TG) mice spontaneously develop HCC at about 1 year of age, providing genetic validation of the oncogenic potential of HBx even in the absence of viral integration and chronic inflammation. Therefore, it would be intriguing to study the regulatory role of HBx in the epigenome and its impact on HCC development. We performed a genome-wide analysis to examine the differences in histone modification and RNA pol II enrichment pattern between cancer and normal liver cells. Overall design: High-throughput sequencing analysis of ChIP (H3, H3K4me3, H3K27me3, H3K36me3, RNA polymerase II) at 3 month old age mouse liver Not all samples in this series have associated SRA records. For samples with unavailable fastq files (GSM2042663, GSM2042665, GSM2042666, GSM2042667), non-fastq formatted sequence files were submitted. These are available as sample supplementary files.