Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [ChIP-seq]

To investigate the genome-wide binding sites of early zygote AP2-family transcription factor, AP2-Z in Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused AP2-Z (AP2-Z::GFP) were harvested at 6 hours post starting ookinete culture (hpoc) and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of AP2-Z were determined from the sequence data. AP2-Z::GFP was subjected to ookinete cultures and harvested at 6 hpoc. Chromatins associated with GFP-tagged AP2-Z was IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control, binding sites of AP2-Z were determined from the sequence data.