Effect of androgen induction and AR depletion on gene expression of metastatic prostate cancer-specific long noncoding RNAs in prostate cancer cells.

We identified 27 long noncoding RNAs with differential expression (DE-lncRNAs) between prostate cancer metastases and corresponding primary tumors, suggesting that they are metastatic castration resistant prostate cancer (mCRPC)-specific lncRNAs. To assess androgen regulation of the DE-lncRNAs, we investigated their expression in LNCaP and VCaP cells induced with dihydrotestosterone (DHT) as well as in androgen receptor (AR)-silenced cells by using RNA-sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data for LNCaP and VCaP cells that were treated with 0 or 10 nM DHT for 24 hours or with AR siRNA or negative control (NC) siRNA for 48 hours. Three biological replicates were sequenced on each treatment type.