Development of organ specific gene expression panel for prediction of organ similarity using transcriptome (KAP220509)

Induced pluripotent stem cell-derived cells might provide a potentially effective approach for both drug development applications and clinical transplantation. Differentiated cells and organoids are expected to provide new cell sources for tissue models and revolutionary clinical therapies. However, conventional experimental methods confirming the expression levels of tissue-specific lineage markers cannot provide complete information regarding the differentiation status or degree of similarity between human tissues and differentiated cell sources. Therefore, in our system, to overcome several issues associated with the assessment of differentiated cells and organoids, we developed a W-SAS (Web-based Similarity Analysis System) algorithm that presents the degree of organ similarity as a percentage. Therefore, our study describes the first quantitative and predictive model for differentiated samples, particularly tissue-specific cells or organoids. And Our W-SAS can provide useful information and insights regarding the differentiation status of in vitro organ models.