Data for: Mouse sperm DNA-methylation changes induced by age and mechanistic target of rapamycin (mTOR) manipulation in Sertoli cells

This study was designed to study changes in sperm DNA methylation induced by age and by changes in activity of two complexes of the mechanistic target of rapamycin (mTOR) in Sertoli cells. Two transgenic mouse models were used. To inactivate mTOR complex one (mTORC1), we used Raptor (component of mTORC1) Sertoli-specific knockout model, and to inactivate mTOR complex two (mTORC2), we used Rictor (component of mTORC2) Sertoli-specific knockout model. Data was collected at two time-pints: postnatal week 8 and postnatal week 22. Sperm DNA methylation of all age/genotype groups was analyzed. Genes associated with differentially methylated regions were identified and biological categories enriched with these genes were identified., To achieve Sertoli-specific knockouts, mice with floxed Rictor or Raptor (Rictortm1.1Klg/SjmJ and B6.Cg-Rptortm1.1Dmsa/J respectively) were crossed with mice with Cre recombinase controlled by the Amh Sertoli-specific promoter (129S.FVB-Tg(Amh-cre)8815Reb/J). All mice were purchased from Jackson Laboratories (Stock ##: 020649, 013188 and 007915 respectively). F3 wildtype and knockout male mice were euthanized at 8 or 22 weeks of age (n = 4 per genotype/timepoint). All procedures followed the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the approval for this study was received from the Institutional Animal Care and Use Committee at University of Massachusetts, Amherst.
At each euthanasia cauda epididymides were incised, cut three times, and incubated at 37C for 30 minutes in 1 ml of sperm wash buffer (Cat. # ART1006, Origio, Denmark). After incubation DNA extraction was done following the rapid method (1). Bisulfite conversion was p..., The dataset contains the following files:
1.     Fastq files for individual RRBS-seq experiments (individual mice) as follows (individual mice ID are included in file names):
Rictor KO, postnatal week 8 – samples M8, M21, M22, and M90
Rictor WT, postnatal week 8 – samples M10, M11, M12, M20
Rictor KO, postnatal week 22 – samples M32, M33, M39, M82
Rictor WT, postnatal week 22 – samples 2i, M19, M38, M81
Raptor KO, postnatal week 8 – samples A1, A2, B1, B20
Raptor WT, postnatal week 8 – samples A10, A20, A22, B21
Raptor KO, postnatal week 22 – samples E22, F1, F10, G2
Raptor WT, postnatal week 22 – samples E20, F2, F20, G1
2.     MS Excel files containing annotated differentially methylated DNA regions form the following comparisons:
WT22_vs_WT8_mTORC1.xlsx – comparison of sperm DNA methylation in 8 and 22 weeks old wildtype mice in mTORC1 experiment.
KO22_vs_WT22_mTORC1.xlsx – comparison of sperm DNA methylation in Raptor knockout and wildtype 22 weeks old mice.
WT22_vs_WT8_mTORC2.xlsx ...