augmentation_data_for_DAISM

The purified dataset for data augmentation for DAISM-DNNXMBD can be downloaded from this repository.

The pbmc8k dataset downloaded from 10X Genomics were processed and uesd for data augmentation to create training datasets for training DAISM-DNN models. pbmc8k.h5ad contains 5 cell types (B.cells, CD4.T.cells, CD8.T.cells, monocytic.lineage, NK.cells), and pbmc8k_fine.h5ad cantains 7 cell types (naive.B.cells, memory.B.cells, naive.CD4.T.cells, memory.CD4.T.cells,naive.CD8.T.cells, memory.CD8.T.cells, regulatory.T.cells, monocytes, macrophages, myeloid.dendritic.cells, NK.cells).

For RNA-seq dataset, it contains 5 cell types (B.cells, CD4.T.cells, CD8.T.cells, monocytic.lineage, NK.cells). Raw FASTQ reads were downloaded from the NCBI website, and transcription and gene-level expression quantification were performed using Salmon (version 0.11.3) with Gencode v29 after quality control of FASTQ reads using fastp. All tools were used with default parameters.