Raw proteomic and metabolomic data

The development of new strategies for articular cartilage regeneration has been investigated, including the use of biomolecules secreted by mesenchymal stem cells (MSCs). We have developed a culture platform based on the use of specific bioreactors for the large-scale culture of from human dental pulp MSCs (hDPSCs) under chondrogenic stimuli. We have optimized secretome production based on known chondral markers (TGF-β1, SERPINE1, and the miRNAs miR-23a, miR-204, miR-211 and miR-337-5p) and have characterized its composition at the metabolomic and proteomic level. We have also studied the chondrogenic potential of the secretome using representative <i>in vitro</i> models. In this study, we developed an MSCs culture system that allows for the production of large volumes of secretome with controlled composition and chondrogenic induction potential. This is a preclinical study necessary for subsequent analysis of the secretome obtained using <i>in vivo</i> experimental models.