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Effect of polyglutamine expanded TBP on microRNA expression in Neuro2A cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE25592
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The study was carried out to identify changes in expression of microRNAs in Neuro2A cells transfected with TATA binding protein (TBP) containing a polyglutamine expansion (59 glutamine-59Q) compared to normal TBP. Neuro2A cells were transfected in triplicate with either 16Q-TBP-GFP fusion or 59Q TBP-GFP Fusion and total RNA collected at 24 hours, 36 hours and 60 hours post-transfection. Each sample was subjected to microRNA microarray analysis as per manufacturer's (Agilent) instructions.
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2012-07-12
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