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Next-generation RNA sequencing of murine bladder cancer cells isolated from P53/PTEN KO tumors at different stages of the disease.. Next-generation RNA sequencing of murine bladder cancer cells isolated from P53/PTEN KO tumors at different stages of the disease.

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Cancer cells from P53/PTEN KO bladder tumors collected at 5, 9, and 13 weeks after adenoviral vector injection. Overall design: Induction of tumor in P53 Fl/Fl PTEN Fl/Fl mice with an adenoviral vector that expresses the Cre-recombinase, isolation of tumor cells by a percoll gradient, in vitro selection of tumor cells, RNA isolation and sequencing.

创建时间:
2019-10-02
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