C57BL/6 DLs hosting live DsRed2 L. am amastigotes showing transcriptional signatures for both DAG and TAG accumulation.

Affymetrix-based analysis detected transcriptional signatures for both DAG and TAG accumulation. Up and down modulations observed between L. am hosting and control DLs are indicated in red and green respectively, for the three pathways leading to DAG/TAG accumulation. Panel A: Different pathways leading to DAG accumulation. A1. Transcriptional signatures accounting for the phosphatidylcholine pathway PC: Phosphatidylcholine, pparγ: peroxysome proliferator-activated receptorγ, pld3: phospholipase D3, ppab2b: phosphatidic acid phosphatase type 2B. A2. Monoacylglycerol pathway MAG: monoacylglycerol, DAG: diacylglycerol, mgat1 and 2: monoacylglycerol O-acetyl transferases 1 and 2. A3. Sn-glycerol-3P pathway G3P: glycerol-3-phosphate, DHA: dihydroxyacetone-phosphate, LPA: Lysophosphatidic Acid, PA: Phosphatidic Acid, sreb: sterol regulatory element binding protein, nf-y: nuclear factor-Y; gpat: glycerol-3-phosphate acyltransferase, pla1: phospholipase A1, pla2: phospholipase A2, pld: phospholipase D, agpat: 1-acyl-glycerol-3-phosphate acyltransferase, dgk: diacylglycerol kinase, lpin 1: lipin-1, lpin2: lipin-2. Panel B: DAG to TAG conversion. abhd5: α/β hydrolase domain-containing protein 5, atgl: adipose triglyceride lipase, dgat1: acyl CoA:diacylglycerol acyltransferase 1, dgat2: acyl CoA:diacylglycerol acyltransferase 2, dgat2L3: diacylglycerol O-acyltransferase 2-like 3, adrp: adipose differentiation related protein, lipa: lysosomal acid lipase A.