DMS-MaPseq: A genome-wide or targeted approach for RNA structure probing in vivo

Here we present dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq), which encodes DMS modifications as mismatches using a thermostable group II intron reverse transcriptase (TGIRT)我们在此介绍硫酸二甲酯测序突变谱分析技术（dimethyl sulfate mutational profiling with sequencing，DMS-MaPseq），该技术借助耐热型II组内含子逆转录酶（thermostable group II intron reverse transcriptase，TGIRT）将硫酸二甲酯修饰位点编码为碱基错配信号。