Mass spectrometry-based proteomics analysis of rat living myocardial slices subjected to cryoinjury.

In this study, we employed living myocardial slices (LMS) as an <i>ex vivo</i> model of cardiac injury to investigate underlying mechanisms and potential therapeutic targets. Cryoinjury was induced in adult rat LMS, resulting in 30% tissue damage. LMS were cultured for 24 hours in biomimetic cultivation chambers while mechanically stretched and electrically stimulated. At the end of the experiment, LMS were split into 2 parts: peri-injury including the injured area plus 1 mm margins, and remote including the rest of the tisse. This proteomics analysis includes three groups: control (uninjured) LMS, injured-peri-injury), and injured-remote. n=3.