Juveniles were dissociated in pools of 12 polyps while adult females were dissociated as individuals. Prior dissociation were labeled for 30 min with EdU (100uM, 2%DMSO) over several time points a
(A-I) Quantification of the number of 253G1, 201B7 and 409B2 cells expanded on laminin-521 in Essential 8 or mTeSR1 medium. Cell numbers were counted every 24 h after plating at 3.2×104 cells/cm2 (A,
