Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of HEK293T cells transfected with the plasmids respectively encoding RfxCas13d, crRNA and NeuN.

To determine what effect the collateral activity of RfxCas13d has on HEK293T cells upon targeting overexpressed NeuN. The plasmids encoding RfxCas13d, NeuN and NT/targeting crRNA into HEK293T cells. After 24 hours of transfection, cells were collected and extracted for total RNA. Then, we performed RNA-seq to compare the differentially expressed genes between cells transfected with targeting crRNAs and non-targeting crRNA. mRNA profiles of HEK293T cells transfected with the plasmids respectively encoding RfxCas13d, NeuN and crRNA.