Additional file 4 of Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors
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Supplementary file 10. Table S1. Primers used to amplify ORFs. The attB regions added upstream are reported below. Kozak CACC sequences on forward primers [46] are indicated in bold. Melting temperatures were calculated by Oligocalc, submitting sequences without attB-regions, and selecting the salt adjusted optimization.
提供机构：
Crava, Cristina M.; Cattaneo, Alberto Maria; Walker, William B.
创建时间：
2025-06-10
