Location and organization of a complex, integrated trangenic array in Caenorhabditis elegans strain hlh-29::gfp

Hairy/Enhancer of Split (HES) proteins play vital roles in animal development and in the regulation of human diseases. The Caenorhabiditis elegans genome encodes six HES orthologs, including HLH-29. We used Nanopore sequencing on the MinION platform to define the location and organization of an integrated trangenic array expressing green fluorescent protein driven by the hlh-29 promoter. The array, which is at least 188.5 kb long, is inserted into Chromosome III of Bristol N2 and contains at least 26 copies of Phlh-29::gfp and 11 copies of rol-6(su1006).