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Single Cell RNAseq profiles of thymic ETP populations

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https://www.ncbi.nlm.nih.gov/sra/SRP221183
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资源简介:
The single cell profiles of sorted ETP populations with cell hashing antibody barcoding from mouse thymus Overall design: Examination of single cell expression profile of sorted populations identified from previous pseudotime analysis. Experiment was performed using droplet-based 3' end RNA expression profiling using Chromium Single Cell 3' Reagent Kits v3 according to manufacturer's protocol (10x Genomics), with the FACS sorted individual sub-populations of ETPs tagged with Totalseq-A hashtags (Biolegend). The cDNA library was sequenced on Illumina Novaseq for 400 million reads, and tag library on HiSeq4000 for 20 million reads, both in pair-end mode following manufacturer's instructions. Please note that meta.csv.gz contains assigned sample names of individual cells after demultiplexing
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2020-01-16
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