DNA accessibility sites requiring SWI/SNF activity in AML [ATAC-seq]

To reveal the genome-wide targets of SWI/SNF complexes in AML cells, we performed ATAC-seq in THP-1, MOLM-13, and MV-4-11 cells with or without the SWI/SNF ATPase BRM014. Analysis of the locations decreased following 24 or 72 h after addition of BRM014 revealed that SWI/SNF-dependent sites are located at enhancers occupied by PU.1, especially the blood enhancer cluster (BENC), a set of enhancers that drives expression of MYC. Overall design: Comparative genome accessibility profiling of AML cells treated with inhibitors of SWI/SNF or PU.1. For comparison to THP-1 differentiation, treatment by phorbol 12-myristate 13-acetate (PMA) was separately assessed.