mRNA target selection from the 30 probes included in the prognostically relevant signature and TaqMan® gene expression assay description.

*Applied Biosystems/Life Technologies.#High inter-run variations of the RQ values for the reference RNA sample (inter-run deltaRQ value range for the reference RNA (19 runs) and for 23 samples (monthly interval between runs) >1.5.∧(1)Complete absence of amplification curves in the reference RNA and/or in all study samples.∧(2)High intra-run variability in deltaRQ values for study duplicates with amplified templates (duplicate deltaRQ >0.8).