Rhythmic transcription drives mRNA level oscillations during C. elegans development [ChIP-Seq]

Thousands of transcripts accumulate in an oscillatory manner during C. elegans larval development. Here, we confirmed these oscillations to result from rhythmic RNA polymerase II (RNAPII) binding to these oscillating genes by performing RNAPII ChIP-seq and total RNA-seq on the same samples (see related submission) Synchronized L1-stage wild-type (N2) worms were plated on food, grown at 25°C and collected hourly from 22 hours until 33 hours developmental time. RNA polymerase II ChIP was performed mouse anti-RNA polymerase II CTD antibody (8WG16, Abcam)