Space flight mouse plasma cell-free RNA seuqnencing in Mouse Habitat Unit-1 (MHU-1)

Purpose: Sequencing analysis of plasma cell-free RNA was performed to study the effects of space flight and microgravity environment in mice. Methods: Plasma samples were collected during the JAXA MHU-1 mission as reported previously (Shiba et al., 2017, Scientific Reports). Total RNA was purified from plasma samples and processed for sequencing analysis, to compare RNA expression profiles in ground control, space flight and artificial 1-G control groups. Overall design: Plasma cell-free RNA profiles were compared among ground control, space flight and artificial 1G control groups.