scRNAseq and scATACseq of Cd45+ cells from brains of 5XFAD mice bearing distinct APOE alleles and across age

Here we analyze the gene expression and chromatin accessibility landscape of immune cells in the brains of 5XFAD mice, a common model of Alzheimer's disease, bearing knock-in of human APOE variants in the mouse APOE locus, and aged to 10, 20, 60, or 96 weeks of age. Cd45+ cells were isolated from hippocampal and cortical regions of brains from 5XFAD APOE-hu/hu mice by fluorescence activated cell sorting (FACS) and analyzed using either scRNAseq or 10X multiome.