DNA and RNA sequencing of single human haploid germ cells

We then sequenced mRNA from single spermatids (302 from the first donor, and 164 from the second), using the Smart-Seq2 protocol. We used these gene expression data, in combination with the haplotype data, to look for gene products that exhibit allele-specific bias reflecting the haploid genotype of the cell. We find a large class of human genes that show such allele-specific bias in spermatids, indicating that these gene products are not fully shared across the cytoplasmic bridges that connect spermatids. We term these genes “genoinformative markers” (GIMs).]]> Discarded testis tissue from deceased male organ donors: Inclusion criteria: Donor age 16 – 80 yrs. Exclusion criteria: Conditions that impede spermatogenesis (e.g. non-obstructive azoospermia. testicular cancer, certain radiotherapies/chemotherapies), sepsis ]]>