Screening archaeological bone for palaeogenetic and palaeoproteomic studies

The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. Please be aware that these samples were processed through two different ancient DNA labs: Bradley Lab at Trinity College Dublin and the Burger Lab at Mainz University. All human and aurochs samples were processed at Mainz and the other animal samples at Trinity. Please see the paper for more details.