Expression data from mouse liver tissue treated by PM2.5 and metformin for two weeks.

There is a lot of epidemiological evidence that PM2.5 causes liver disease, and metformin can alleviate these diseases.However, the precise mechanism remains unclear.Our study defined the dynamic changes of the differentially expressed genes related PM2.5 induced diseases, and provide basis for comprehensive understanding of the mechanism at the transcriptome level. We investigated PM2.5 induced gene expression alteration associated with the development of liver injury by microarray in a ob/ob mouse model. Mice were euthanized after two weeks PM2.5 exposure. Total RNA was isolated with TRIzol (Invitrogen) from liver organs (n=5 per group) at each time point according to manufacturer’s instructions. Gene expression profiling was performed using Affymetrix GeneChip mouse Genome 430 2.0 array according to the manufacturer’s instructions (Affymetrix, Inc., Santa Clara, CA). On the GeneChip Mouse Genome 430 2.0 Array, over 45,000 probe sets analyze the expression level of over 39,000 transcripts and variants from over 34,000 well characterized mouse genes.