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Rhizobium leguminosarum symbiovar trifolii nodule samples PCR processed with different polymerases

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA596932
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资源简介:
Rhizobium leguminosarum symbiovar trifolii (Rlt) nodule samples collected from Store Heddinge, Denmark. These samples were used to validate a metabarcoding method using amplicons with unique molecular identifiers to improve error correction: MAUI-seq. Raw read files contain pooled amplicon sequences of Rlt genes rpoB, recA, nodA and nodD. Samples were PCR processed independently with Platinum (non-proofreading) and Phusion (proofreading) polymerases. This was to evaluate the MAUI-seq method dependency on polymerase choice.
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2019-12-20
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