Long-term survival after cryopreservation of the endangered exceptional species Crotalaria avonensis. Philpott et al., 2025.

Data for publication, "Long-term survival after cryopreservation of the endangered exceptional species Crotalaria avonensis". Data represents long- and short-term survival after cryopreservation for the endangered plant species, Crotalaria avonensis. This study presents an assessment of survival across three variations on the encapsulation vitrification method for 3 – 16 years of cryostorage for shoot tips of the federally endangered exceptional plant species, C. avonensis. In addition, the more recently-developed droplet vitrification method was compared to encapsulation vitrification for short-term survival. Overall, over 92% of banked genotypes survived after long-term cryo-storage, and the droplet vitrification method appears to improve survival over the original encapsulation vitrification procedures. In addition, the long-term survival data validate the previously published guidance that a 40% initial survival after liquid nitrogen exposure will lead to a 95% chance of survival after long-term storage, with 39% initial survival in C. avonensis leading to a 95% probability of survival after long-term storage per genotype. Initial survival after short-term liquid nitrogen exposure appears to be an indicator of long-term survival in storage, which can give practitioners some indication of their banking success.