HA and NA properties of H1N1pdm HA222 variants.

aDissociation constant Kd of the viruses for 6′SLN sialylglycopolymer (Neu5Acα2-6Galβ1-4GlcNAc-PAA-Biotin) and 3′SL sialylglycopolymer (Neu5Acα2-3Galβ1-4Glc-PAA-Biotin) were calculated using the non-linear one site specific binding equation using the GraphPad software. The values are the mean of two assays. bMichaelis Menten constants (Km) were determined with MU-NANA ranging from 10 to 200 µM. Results are given as the mean Km values calculated using values derived from three separate assays. cNeuraminidase inhibition assays (IC50) were performed on cell supernatants with MU-NANA as a substrate at a final concentration of 50 µM. Values were obtained from duplicate samples.