Akt binding partners identified from Neuro 2A cells using a two-step crosslinking-co-IP/MS approach.

#None of these proteins were detected without using DSP-crosslinking, with the exception of ERK1/2. Without DSP-crosslinking ERK1/2 was identified by only two distinct peptides (G[14]–[22]R and A[193–201]K) with 5% sequence coverage.aProteins found to be more associated with active Akt than inactive Akt by western blot analysis.bAssociation with Akt found to be independent of the activation status of Akt revealed by western blot analysis.cpeptides identified by Bioworks (3.1).dXC score.