miRNA and gene expression profiling in human thyroid carcinomas and non-neoplastic thyroids [miRNA]

We performed miRNA and gene expression profiling in a series of 30 thyroid carcinomas and 6 non-neoplastic thyroids. MiRNA and gene expression profiles were established by microarray analysis in a series of 36 snap-frozen tissues using SurePrint G3 Human miRNA 8x60K microarrays (Agilent Technologies) and HumanHT-12 WG-DASL V4.0 R2 expression beadchip (Illumina), respectively. Tissue samples were obtained from Fondazione IRCCS Istituto Nazionale dei Tumori (Milan) and include various thyroid carcinoma histotypes: 20 papillary carcinomas (PTCs) consisting of different histological variants, 7 poorly differentiated thyroid carcinomas (PDTCs) and 3 lymph node metastases derived from PTC.