Long-read cDNA sequencing for human stem-cell derived retinal cells

The purpose of this study was to perform long-read sequencing (LRS) on differentiating 2D and 3D retinal stem cell cultures to analyze expression of mRNA isoforms in different retinal cell populations.Our LRS data can be used to quantify isoform expression in different retinal cell populations in differentiated 2D cultures and 3D ROs. We are currently comparing isoform expression across RO cell types/time points, as well as 2D stem cell-derived RGC and RPE cultures. Ongoing experiments are characterizing alternative splicing patterns in both healthy and disease states through differentiation of mutant stem cell lines.