Aspergillus flavus gene expression profile under different conditions of ustiloxin production

Transcriptome data of A. flavus was assembled under four different conditions (two strains and two media) for omics data analyses of genes related to ustiloxin production. The used two strains were the over-expressed strain (ustR-oe) of the transcription factor in ustiloxin gene cluster, ustR, and the pyrG marker complemented strain (control). The two media were V8 juice liquid and potato dextrose broth (PDB) media. The ustR-oe strain produced more ustiloxin B than the control strain, and the production is much higher in V8 than in PDB. Therefore, four different state of transcriptome could be obtained under these four conditions. The obtained transcriptome data was combined with the GEO data acc. no. GSE15435 and uesd for omics data analyses. 1E5 spores of ustR-oe and control strains were inoculated to 30 mL of V8 and PDB media in 100-mL flask with buffle and cultured for 2 days at 30C, 165 rpm. The mRNA was extracted and labeled according to the manufacturer's protocol. Two biological replicates were taken for each sample. The raw data was normalized (Z-scored) using Agilent GeneSpring software.