Quercetin nanocrystals prepared by a novel technique improve dissolution rate and antifibrotic activity of quercetin - Supplementary materials

Aim: To develop quercetin nanocrystals by a simple approach and to evaluate their in vivo antifibrotic
efficacy. Materials & methods: Nanosuspensions were fabricated by a thin-film hydration technique and
ultrasonication. The influence of process variables on the average diameter of quercetin nanoparticles was
investigated. Moreover, in vivo efficacy was investigated in an established murine CCl4-induced fibrosis
model. Results: Nanocrystals showed a particle size of <400 nm. The optimized formulations showed an
increase in dissolution rate and solubility. Quercetin nanocrystals markedly prevented fibrotic changes in
the liver, as evidenced by mitigated histopathological changes and diminished aminotransferase levels and
collagen accumulation. Conclusion: The findings reflect the promising potential of quercetin nanocrystals
for liver fibrosis prevention.