A microRNA expression signature of osteoclastogenesis (CBX146). unidentified
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https://www.ncbi.nlm.nih.gov/bioproject/PRJDB20113
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Primary mouse bone marrow-derived monocyte/macrophage precursors (BMMs) were prepared from the femur and tibia of 4-to-6 week old mice and cultured with 20 ng/ml M-CSF in the absence (control) or presence of 100 ng/ml RANKL for 24 h. Total RNA with small RNA (< 200 nucleotides) was isolated using the mirVana? miRNA isolation kit (Ambion, Austin, TX) following the manufacturer?fs suggestion and immediately frozen in dry ice and submitted to LC Sciences (Houston, TX). Dual-color hybridization microarray studies for miR expression were performed using the LC Science array platform containing probes for 617 mature mouse miRs present in miRBase.
创建时间：
2025-01-20
