GNPS - NASTE DOM and coral tissue nutrient dosing experiment

Dissolved organic matter and coral tissue metabolites from a three, three week nutrient dosing experiment using an inorganic nutrient mixture, bird guano, or effluent. DOM and tissue metabolites were from either Porites or Montipora coral treatments. The first three weeks just dosed the corals with nutrients, the following increased temperatures to simulate future warming conditions, and the last three were a recovery period. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 150 x 2.1 mm (Phenomenex, Torrance, CA).