Effects of EDTA and sodium sulfite on R. padi transmission of purified cereal yellow dwarf virus-RPV using microinjection into the aphid hemolymph.

aAphids were microinjected with purified luteovirus. For each experiment, the concentration of virus the same. Among different experiments, virus concentration varied from 20–60 µg/ml. Five experiments were performed using no additives, 2 experiments were performed using both sodium sulfite and EDTA, and 3 experiments were performed using only EDTA or sodium sulfite.bFor each treatment, the numbers represent total number of plants infected/total number of plants inoculated. Three aphids previously microinjected with virus were placed on each healthy plant.