TMT-based quantitative proteomics analysis reveals the effects of the α-lactalbumin peptides GINY and DQW on lipid deposition and oxidative stress in HepG2 cells

To further explore the underlying mechanism of the α-lactalbumin peptides Gly-Ile-Asn-Tyr (GINY) and Asp-Gln-Trp (DQW) in alleviating hepatic steatosis, TMT-based quantitative proteomics was performed. Overall, a total of 3808 quantifiable proteins were identified from the four groups, and the quantitative results were visualized in Table S1. Among them, 213 proteins were identified as DEPs (Tables S2-4). Further analysis showed that 37 proteins were the mutual DEPs among the four groups (Table S5). As shown in Fig. 2b and Table S2, 155 proteins were identified as DEPs in the FFAs group (versus the control group). Compared with the FFAs group, 99 proteins were identified as DEPs in the GINY group (Table S3), and 89 proteins were identified as DEPs in the DQW group ( Table S4).