Bovine sperm surface lectinome

Identification of bovine sperm surface carbohydrate-binding proteins relevant in two important aspects of fertilization: (i) formation of the sperm reservoir in the oviductal epithelium, and (ii) gamete recognition (oocyte-sperm interaction). Using whole sperm cells and a novel affinity capture method that combines proteolysis of protein-glycan complexes and mass spectrometry (i.e., CREDEX-MS), several lectins were enriched, identified by MS/MS proteomics, and mapped within the fertilization events in bovine species.