RNA-seq of human K562 leukemia cells with or without a mutation in SF3B1 (splicing factor 3b, subunit 1) that were treated with DMSO or the STAT3 inhibitor STATTIC

The purpose of the experiment was to define the gene expression and splicing alterations occurring when human wildtype or splicing factor 3b subunit 1 (SF3B1) mutant K562 cells had STAT3 inhibited with the small molecule STATTIC. Results provide insight the interplay of SF3B1 and STAT3 in human hematopoietic cells. Overall design: mRNA profiling of human K562 cells with or without a mutation in SF3B1 (K666N) treated with DMSO or the STAT3 inhibitor STATTIC using a NovaSeq6000. A total of 18 samples were sequenced.