RNA-sequencing of human embryonic stem cell-derived retinal pigment epithelial cells (hESC-derived RPE) at different time points of culture.

We report that the phase/stage of cell culture used for cryopreservation greatly affects the post-thaw outcomes. The goal of the study is to compare the transcriptome profiles between different time points and uncover the mechanism underlying RPE tolerace to freezing. Overall design: In this study, hESC-derived RPE cells were collected and seeded at passage 1 day 35 (P1D35). After passage, these cells were labeled as passage 2 (P2).Three independent batches of hESC-derived RPE cells at P1D35, P2D5 and P2D11 (9 samples in total) were subjected to RNA sequencing.