The composition and nutrient content of the basal diet.

1Crude protein and crude lipid contents were measured value. Available phosphorus, n-3 and n-6 contents were calculated value.2Amino acid mix (g/kg): lysine, 17.13 g; methionine, 7.78 g; tryptophan, 3.57 g; threonine 11.88 g; arginine, 12.89 g; histidine, 7.96 g; leucine, 20.51 g; phenylalanine, 13.60 g; valine, 15.33 g; cystine, 0.91 g; tyrosine, 10.86 g; glutamic acid, 32.32 g.3L-isoleucine was added to obtain graded levels of isoleucine. Per kilogram of isoleucine premix composition from diet 1 to 6 was as follows (g/kg): L-isoleucine 0.00, 60.86, 121.71, 182.57, 243.42, 304.28 g and corn starch 1000.00, 939.14, 878.29, 817.43, 756.58, 695.72, respectively.4Glycine premix: Each mixture was made isonitrogenous with the addition of reduced amounts of glycine and compensated with appropriate amounts of corn starch. Per kilogram of glycine premix composition from diet 1 to 6 was as follows (g/kg): 720.96, 699.49, 678.03, 656.57, 635.10, 613.64 g and corn starch 279.04, 300.51, 321.97, 343.43, 364.90, 386.36 g, respectively.5Per kg of vitamin premix (g/kg): retinyl acetate (500 000 IU/g), 0.80 g; cholecalciferol (500 000 IU/g), 0.48 g; DL-α-tocopherol acetate (500 g/kg), 20.00 g; menadione (230 g/kg), 0.22 g; cyanocobalamin (10 g/kg), 0.10 g; D-biotin (20 g/kg), 5.00 g; folic acid (960 g/kg), 0.52 g; thiamine hydrochloride (980 g/kg), 0.12 g; ascorhyl acetate (930 g/kg), 7.16 g; niacin (990 g/kg), 2.58 g; meso-inositol (990 g/kg), 52.33 g; calcium-D-pantothenate (900 g/kg), 2.78 g; riboflavin (800 g/kg), 0.99 g; pyridoxine hydrochloride (980 g/kg), 0.62 g. All ingredients were diluted with corn starch to 1 kg.6Per kg of trace mineral premix (g/kg): FeSO4.H2O, 25.00 g; CuSO4.5H2O, 0.60 g; ZnSO4.H2O, 4.35 g; MnSO4.H2O, 2.04 g; KI, 1.10 g; NaSeO3, 2.50 g; MgSO4.H2O, 230.67 g. All ingredients were diluted with corn starch to 1 kg.The composition and nutrient content of the basal diet.