6K-plex RNA CosMx data from DLBCL sections.

Diffuse large B-cell lymphomas (DLBCLs) are a common and heterogeneous group of mature B-cell malignancies that typically arise in lymph nodes; sites in which stromal and endothelial cells, antigen-presenting cells and other myeloid cells facilitate adaptive immune responses by T-cells and B-cells against pathogens. In this study, we have leveraged the spatial transcriptomic platform of CosMx together with CODEX imaging technology to investigate the spatial immunology and pathobiology of DLBCL. CosMxTM technology [CosMxTM Human Universal Cell Characterization RNA Panel (1000-plex) + 20 custom genes; Bruker Spatial, USA] was applied to 6 tissue microarrays (TMAs i.e, TMA DLBCL 1, TMA DLBCL 2, TMA DLBCL 3, TMA DLBCL 4, TMA DLBCL 5, TMA DLBCL 6 ) from excisional biopsies of 78 large B-cell lymphomas and 5 controls (4 tonsil, 1 lymph node). Large B-cell lymphomas included 66 DLBCL not otherwise specified (DLBCL NOS); 5 EBV+ DLBCL NOS; 4 T-cell/histiocyte-rich large B-cell lymphoma (TCHRBCL); 2 primary mediastinal large B-cell lymphoma (PMBCL); and 1 post-transplant lymphoproliferative disease (PTLD). Note: The raw data is common for all the samples in the corresponding TMA and the corresponding FOV for each sample has been described in the Supplementary File or can be requested from the contributor.